<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jordaens, K.</style></author><author><style face="normal" font="default" size="100%">Sonet, G.</style></author><author><style face="normal" font="default" size="100%">Richet, R.</style></author><author><style face="normal" font="default" size="100%">Dupont, E.</style></author><author><style face="normal" font="default" size="100%">Braet, Y.</style></author><author><style face="normal" font="default" size="100%">Desmyter, S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Identification of forensically important Sarcophaga species (Diptera: Sarcophagidae) using the mitochondrial COI gene</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Legal Medicine</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">coi</style></keyword><keyword><style  face="normal" font="default" size="100%">diptera</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Barcoding</style></keyword><keyword><style  face="normal" font="default" size="100%">Forensic entomology</style></keyword><keyword><style  face="normal" font="default" size="100%">identification</style></keyword><keyword><style  face="normal" font="default" size="100%">Sarcophagidae</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">03/2013</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">127</style></volume><pages><style face="normal" font="default" size="100%">491-504</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The identification of species of the forensically important genus &lt;em&gt;Sarcophaga &lt;/em&gt;is very difficult and requires strong taxonomic expertise. In this study we sequenced the mitochondrial cytochrome c oxidase subunit I gene (COI) of 126 specimens of 56 W European &lt;em&gt;Sarcophaga &lt;/em&gt;species. As such we established the first COI database for West European &lt;em&gt;Sarcophaga&lt;/em&gt;. We added existing data to our database to yield a total dataset of 270 COI sequences from 99 species to evaluate the COI gene as a molecular diagnostic tool for species identification in this genus. Using two simple criteria (Best Match and Best Close Match) we showed that the identification success using a mini-barcode region of 127 bp was very low (&amp;lt;61%). The identification success using the standard barcode region (658 bp) or using the entire COI region (1535 bp) was similar but relatively low: 75-80%. This was mainly due to specimens that were only represented by a single sequence in the dataset. Omitting these species resulted in a strong increase of the identification success: 96.9-98.5%. This success was even further increased when applying a nucleotide-based analysis but the latter may not be suitable for decision making. Even though the COI gene seems suitable to discriminate among most &lt;em&gt;Sarcophaga &lt;/em&gt;species, additional markers will be necessary to achieve a 100% identification success.&lt;/p&gt;
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