02682nas a2200349   4500008004100000245006900041210006800110300001100178490001500189520164000204653000901844653002901853653001501882653001401897653000801911653001401919653002701933653001501960653001501975653001801990653001302008653002702021653002902048653003302077653002802110653001102138100001602149700002702165700001802192700002202210856010002232       2009                        eng d00aDNA barcode accumulation curves for understudied taxa and areas.0 aDNA barcode accumulation curves for understudied taxa and areas  a208-160 v9 Suppl s13 aFrequently, the diversity of umbrella taxa is invoked to predict patterns of other, less well-known, life. However, the utility of this strategy has been questioned. We tested whether a phylogenetic diversity (PD) analysis of CO1 DNA barcodes could act as a proxy for standard methods of determining sampling efficiency within and between sites, namely that an accumulation curve of barcode diversity would be similar to curves generated using morphology or nuclear genetic markers. Using taxa at the forefront of the taxonomic impediment - parasitoid wasps (Ichneumonidae, Braconidae, Cynipidae and Diapriidae), contrasted with a taxon expected to be of low diversity (Formicidae) from an area where total diversity is expected to be low (Churchill, Manitoba), we found that barcode accumulation curves based on PD were significantly different in both slope and scale from curves generated using names based on morphological data, while curves generated using nuclear genetic data were only different in scale. We conclude that these differences clearly identify the taxonomic impediment within the strictly morphological alpha-taxonomy of these hyperdiverse insects. The absence of an asymptote within the barcode PD trend of parasitoid wasps reflects the as yet incomplete sampling of the site (and more accurately its total diversity), while the morphological analysis asymptote represents a collision with the taxonomic impediment rather than complete sampling. We conclude that a PD analysis of standardized DNA barcodes can be a transparent and reproducible triage tool for the management and conservation of species and spaces.10a200910aaccepted 27 january 200910aBraconidae10achurchill10aco110acynipidae10acytochrome c oxidase 110adiapriidae10aformicidae10aIchneumonidae10amanitoba10aphylogenetic diversity10areceived 22 october 200810arevision received 16 january10aspecies abundance curve10atriage1 aSmith, Alex1 aFernandez-Triana, J.L.1 aRoughley, Rob1 aHebert, Paul, D N  uhttp://seag-insectes.com/en/content/dna-barcode-accumulation-curves-understudied-taxa-and-areas